Evaluation of the Murex Hybrid Capture Cytomegalovirus DNA Assay versus Plasma PCR and Shell Vial Assay for Diagnosis of Human Cytomegalovirus Viremia in Immunocompromised Patients
نویسندگان
چکیده
منابع مشابه
Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
متن کاملComparison of cytomegalovirus antigenemia assay with shell vial-indirect immunofluorescence assay for rapid detection of cytomegalovirus in blood.
The recent article by Mazzulli et al. (4) further demonstrates the increasing interest of virologists and microbiologists in the cytomegalovirus antigenemia (CMV-Ag) assay. Mazzulli and coworkers report that the CMV-Ag assay is more sensitive than the shell vial-indirect immunofluorescence assay (SVAIFA), perhaps implying that the direct antigen detection assay should be considered the method o...
متن کاملMulticenter comparison of the digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of cytomegalovirus viremia.
We compared the Digene Hybrid Capture CMV DNA Assay version 2.0, the pp65 antigenemia assay, traditional tube culture, and shell vial culture for the detection of cytomegalovirus (CMV) viremia in several patient populations at three centers. Of 561 blood specimens collected from 402 patients, complete clinical and laboratory data were available for 489. Using consensus definitions for true posi...
متن کاملdevelopment of a sensitive quantitative competitive pcr assay for detection of human cytomegalovirus dna
accurate and rapid diagnosis of human cytomegalovirus (hcmv) disease in immunocompromised patients has remained as a challenge. quantitative competitive pcr (qc-pcr) methods for detection of hcmv in these individuals have improved the positive and negative predictive values of pcr for diagnosis of hcmv disease. in this study we used qc-pcr assay, using a co-amplified dna standard, to quantitate...
متن کاملA real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient
Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1998
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.36.9.2554-2556.1998